Short Course

12th November, 2017

9:00 - 12:30

Prof. Gary Hieftje (Department of Chemistry, Indiana University)
Course Title : Glow Discharge - The Most Versatile Source
Highlight : The broad range of designs, operating conditions, and capabilities of glow discharges will be reviewed, and areas of application will be considered. Newer designs and capabilities will be emphasized, but historical aspects will also be covered.
Keyword : fundamentals, instrumentation, glow discharges
Dr. Stephan Chady (PerkinElmer) and
Prof. Ruth Merrifield (Center for Environmental Nanoscience and Risk, Univ. of South Carolina)
Course Title : Trends in Single Particle and Single Cell ICP-MS: From particles detection in complex matrices to quantification of particle number and metal content in individual unicellular organisms
Highlight : This course will briefly review the theory of SP-ICP-MS focusing on the state-of-the-art innovations in hardware and software with relation to the latest applications in Environmental, Forensic and Semiconductor sciences. We will introduce the concept of Single Cell-ICP-MS and discuss the challenges faced when analyzing cells compared to NPs with an in-depth focus on the necessary validation required to accurately quantify the number of particles and/or metal content in individual unicellular organism. The implications to human and environmental health applications of this technique will be discussed: Cisplatin uptake by cancer cell, nanoparticles uptake and transformation by fresh water algae and intrinsic metal quantification.
Keyword : Single Cell ICP-MS, Single Particle ICP-MS, Bioaccumulation, Nanoparticles, Uptake, Environmental, Cancer, Semiconductor, Forensic, Algae
Prof. Joerg Feldmann (Trace Element Speciation Laboratory, University of Aberdeen)
Course Title : Arsenic and mercury speciation in biological samples
Highlight : In this talk I will give some insights into the target analysis of inorganic arsenic or methylmercury in rice but also will discuss the non-targeted approach for the identification of all organoarsenicals in plants and in marine samples in order to determine novel organoarsenic compounds such as the arsenolipids or mercury phytochelatins.
Keyword : speciation, environments

13:30 - 17:00

Prof. Takafumi Hirata (Geochemical Research Center, The University of Tokyo)
Course Title : Laser ablation sample introduction technique : principles, system setup and applications for imaging on geochemical and biochemical materials (in Japanese)
Outline : The laser ablation-inductively coupled plasma mass spectrometry (LA-ICPMS) is widely accepted as one of the most advanced, sensitive and rapid analytical tools for elemental and isotopic analysis of solid materials.  With the LA-ICPMS technique, direct elemental and isotopic analyses can be made without chemical decomposition or dissolution procedures. Moreover, analytical capability for in-situ elemental and isotopic analyses can be achieved in various spatial resolution, ranging from 1 - 1,000 µm. The LA-ICPMS technique has blossomed to become the key technique for both the in-situ isotope-dating and mapping analysis of trace-elements (imaging mass spectrometry). The short course will cover from principles to latest applications of the LA-ICPMS technique. Note that the courses will be made with Japanese language.
Keyword : laser ablation, instrumentation, imaging, nano-particle
Prof. Ryszard Lobinski (Institute of Analytical Sciences and Physical Chemistry - IPREM, CNRS, Pau, France)
Course Title : Element speciation in life sciences and metallomics
Highlight : The understanding of the molecular mechanisms of metal-dependent life processes requires fine analytical chemistry approaches including ultratrace element determination, spatial imaging, precise isotope ratio measurement and speciation (analysis for individual metal species). In recent years, each of these approaches has grown into a separate field of research and development with a number of specific and complementary applications. The development of techniques and methods for the probing of interactions between metal ions and the organism's genome and the derived -omes: proteome and metabolome resulted in the emergence of the field of metallomics.
This short course discusses the fundamentals and state-of-the art of mass-spectrometry based analytical techniques to probe for metal species in biological cells and tissues.
Keywords : biological trace element analysis, speciation, metallomics
Prof. Frank Vanhaecke (Department of Analytical Chemistry, Ghent University)
Course Title : An introduction to isotopic analysis with single- and multi-collector ICP-MS
Highlight : As a mass spectrometric technique, ICP-MS provides information on the isotopic composition of the elements. As in a first approximation, it can be stated that the isotopic composition of the elements is invariant in nature, a first type of applications relies on induced changes in the isotopic composition as a result of the use of (a) stable enriched isotope(s). These applications include elemental assay via isotope dilution and tracer experiments. However, this statement has to be refined - in fact, all elements with ≥ 2 isotopes show some natural variation in their isotopic composition. The sources of this natural variation will be discussed. Specific attention will be devoted to elements with radiogenic nuclides (e.g., Sr, Pb) and to mass-dependent and mass-independent isotope fractionation. It will be shown how the extent of this variation determines the type of ICP-mass spectrometry (single- or multi-collector) that can be deployed. The importance of isotope ratio precision will be underlined and hints for optimization of the instrument settings and data acquisition parameters aiming at an optimum isotope ratio precision will be provided for single- and multi-collector ICP-MS. Correction for detector dead time (pulse counting detectors) and for instrumental mass discrimination, required to convert the raw measurement data into "true" isotope ratios, will be discussed. Examples of applications relying on the determination and quantification of natural isotope ratio variation will include provenance determination of agricultural products, environmental applications, geo-/cosmochemical applications (including geochronological dating) and the use of isotopic analysis for medical diagnosis.
Keywords : isotope ratio, isotope fractionation, radiogenic nuclide, precision, MC-ICP-MS